General Cell Lines and Cell Cultures
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Small Intestine, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx PathHunter U2OS mHTR2C β-Arrestin Cell Line, 2 vials. (93-0790C3)
The PathHunter mHTR2C cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of mHTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mHTR2C and EA-tagged β-Arrestin-2. Activation of mHTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Section - Human Tumor: Bone, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Eurofins Discoverx PathHunter C2C12 CXCR4 β-Arrestin Cell Line, 2 vials. (93-0203C7)
The PathHunter CXCR4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. [ligand]) based activation of CXCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR4 and EA-tagged β-Arrestin-2. Activation of CXCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Genscript Corporation CHO-K1/G15 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human G protein subunit alpha 15 (G 15) in the cytoplasm This protein enables G protein-coupled receptor binding activity and acts upstream of or within with a positive effect on calcium-mediated signaling
ATCC Calu-6; Anaplastic Carcinoma; Human (Homo sapiens)
Calu-6 is a cell line exhibiting epithelial morphology that was derived from a 61-year-old, White female patient with anaplastic carcinoma. This product has applications in cancer research.
Eurofins Discoverx PrecisION hnAChR α7/ric3 Recombinant Stable Cell Line, 2 vials. (CYL3097)
The PrecisION hnAChR α7/ric3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying nAChR α7/ric3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses nAChR α7/ric3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx PathHunter U2OS CXCR3 β-Arrestin Cell Line, 2 vials. (93-0271C3)
The PathHunter CXCR3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiBrite EP1 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS099L)
The ChemiBrite EP1 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER1, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay PK2 Prokinecticin Receptor Frozen Cells, 2 Vials of Cells. (HTS182RTA)
The Ready-to-Assay PK2 Prokinecticin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PROKR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen BB1 Bombesin/Neuromedin B Receptor Membrane Preparation, 1 vial. (HTS123M)
The ChemiScreen BB1 Bombesin/Neuromedin B Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of NMBR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC LL/2 (LLC1); Lewis Lung Carcinoma; Mouse (Mus musculus)
LL/2 (LLC1) Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma. This cell line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.
ATCC U-87 MG-Luc2; Glioma; Human (Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
ATCC Mouse STR Profiling Service
FTA Sample Collection Kit for Mouse Cell Authentication Service utilizes short tandem repeat (STR) profiling. Trained experts amplify 18 STR loci plus 2 specific loci for detection of human or African green monkey species within the submitted sample. A comprehensive analysis report interprets both karyotypically normal and abnormal cell lines, includes an electropherogram supporting the allele calls at each locus, known reference profiling against the ATCC Mouse STR database and a comprehensive interpretation of results. Sample kit mailed upon purchase. Download the instructions for sample preparation and submission. This service is performed according to Almeida JL, et al. Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines. PLoS ONE 14(6): e0218412, 2019. PubMed: 31220119.